A rpoN knockout mutant strain of P. aruginosa PA01UNSW was created to test the effect of RpoN on the expression of rhlI. A lacZ fusion assay determined that RpoN positively regulates rhlI transcription, demonstrating its role as an activator of rhIl. Alignment of promoter of genes known to be regulated by RpoN identified an RpoN consensus sequence (YTGGCACGrNNNTTGCW).
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|