Curation Information

Publication
A single nucleotide change affects fur-dependent regulation of sodB in H. pylori.;Carpenter BM, Gancz H, Gonzalez-Nieves RP, West AL, Whitmire JM, Michel SL, Merrell DS;PloS one 2009; 4(4):e5369 [19399190]
TF
Fur [Q1CU85, view regulon]
Reported TF sp.
Helicobacter pylori HPAG1
Reported site sp.
Helicobacter pylori HPAG1
Created by
Grace Chandler
Curation notes
-

Experimental Process

RNase protection assays and visual sequence inspection of the sodB promoter determined a single nucleotide polymorphism at the -5 bp affects the regulation of sodB by apo-Fur in H.pylori G27, H.pylori 26695, H.pylori J99, and H.pylori HPAG1. EMSAs confirmed that apo-Fur binds specifically to the sodB promoters in H.pylori G27 and H.pylori 26695.

Transcription Factor Binding Sites


AAGAAAAGATTTACCAAAAAGTATTAAAAAATGATTACAATAC
AAGAAAAGATTTACCAAAAAGTATTAAAAAATGATTACAATAC

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
AAGAAAAGATTTACCAAAAAGTATTAAAAAATGATTACAATAC HPAG1_1003,
... ... HPAG1_1003 HPAG1_1002 HPAG1_1004
Experimental technique details EMSA (ECO:0001807) - Experimental technique details RNAse protection (ECO:0000288) - Experimental technique details Visual sequence inspection (nan) - not specified not specified