RNase protection assays and visual sequence inspection of the sodB promoter determined a single nucleotide polymorphism at the -5 bp affects the regulation of sodB by apo-Fur in H.pylori G27, H.pylori 26695, H.pylori J99, and H.pylori HPAG1. EMSAs confirmed that apo-Fur binds specifically to the sodB promoters in H.pylori G27 and H.pylori 26695.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|