Curation Information

Direct interaction between quorum-sensing regulator SmcR and RNA polymerase is mediated by integration host factor to activate vvpE encoding elastase in Vibrio vulnificus.;Jeong HS, Kim SM, Lim MS, Kim KS, Choi SH;The Journal of biological chemistry 2010 Mar 26; 285(13):9357-66 [20110369]
SmcR [Q7MHU7, view regulon]
Reported TF sp.
Vibrio vulnificus YJ016
Reported site sp.
Vibrio vulnificus YJ016
Created by
Alex Mak
Curation notes

Experimental Process

Cross-linked chromatin from the wild type and smcR mutant HS03 cells was immunoprecipitated using the antibody against SmcR.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... VVA1465 VVA1466 VVA1467
Experimental technique details ChIP-PCR (ECO:0005620) - Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - Experimental technique details Site directed mutagenesis (ECO:0005667) - activator dimer