Beta-gal fusion assays demonstrated that Fur directly regulates the irr gene as a threefold repression of irr occurred when the fur gene was introduced. EMSA confirmed Fur binding to the irr promoter.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
| Site sequence | Regulated genes | Gene diagram | Experimental techniques | TF function | TF type |
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