EMSA was used to demonstrate Fur binding to the irr promoter. DNase I footprinting localized the Fur binding region in the irr promoter. In vitro transcription assay showed that Fur represses irr expression.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|