Curation Information

Fructose 1-phosphate is the preferred effector of the metabolic regulator Cra of Pseudomonas putida.;Chavarría M, Santiago C, Platero R, Krell T, Casasnovas JM, de Lorenzo V;The Journal of biological chemistry 2011 Mar 18; 286(11):9351-9 [21239488]
Cra [Q88PQ6, view regulon]
Reported TF sp.
Pseudomonas putida KT2440
Reported site sp.
Pseudomonas putida KT2440
Created by
Dinara Sagitova
Curation notes

Experimental Process

A putative Cra binding site was identified by searching P. putida KT2440 genome with an E.coli Cra binding motif. EMSA and the isothermal titration calorimetry experiments confirmed Cra binding to its target site.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... fruR PP_0791 fruB fruK fruA
Experimental technique details EMSA (ECO:0001807) - Experimental technique details Isothermal titration calorimetry (ECO:0005647) - Experimental technique details PSSM site search (ECO:0005659) - not specified dimer