Curation Information

Activation and repression of a sigmaN-dependent promoter naturally lacking upstream activation sequences.;Porrúa O, García-González V, Santero E, Shingler V, Govantes F;Molecular microbiology 2009 Aug; 73(3):419-33 [19570137]
RpoN [P0A171, view regulon]
Reported TF sp.
Pseudomonas sp. ADP
Reported site sp.
Pseudomonas sp. ADP
Created by
Dinara Sagitova
Curation notes

Experimental Process

In vitro transcription was shown to be RpoN dependent. EMSAs showed that AtzR binds the PatzR promoter and that it competes with sigma-N for binding. This was further confirmed by DNAse footprinting.

The paper reports that TF forms complex with other proteins for binding with reported sites.

AtzR and Sigma-N compete for binding, leading to PatzR repression.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... orf99 orf94 orf95 orf96 orf97 orf98 atzD
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details In-vitro transcription (ECO:0001204) - activator monomer