EMSA in the presence of fhuDC and fur promoters. DNAse I protection assay and comparative analysis to identify the protected region (on fur promoter only). Northern blot analysis to study the effect of iron on fur transcription.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|