Started by performing a full-genome search of the binding motif (G/CAAAC N2-4 GTTTG/C), with the variance of N used to investigate the importance of spacing between the two motifs and maximize number of candidate genes. Of the results, 10 were deemed probable and tested via Beta-gel reporter assay, which demonstrated that 4 of the genes were regulated by PsrA. 2D-PAGE was used to identify differences in expression pattern between the wild type and a strain lacking PsrA, and identified 11 protein spots for investigation. Following EMSA of 3 identified genes and a control, all 3 were shown to bind with PsrA.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|