Transcription start points determined by primer extension. In silico search for consensus sequence in promoter region using MyPatternFinder (http://www.nii.ac.in/~deepak/MyPattern/) found 6 high scoring sites not previously reported in literature. Expression measured with GFP fusion. Western blot to confirm expression. EMSA to demonstrate binding of DevR using entire promoter region. binding sites NOT sequenced -- putative binding sites from computational analysis assumed to bind.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|