Curation Information

Publication
PsrA, the Pseudomonas sigma regulator, controls regulators of epiphytic fitness, quorum-sensing signals, and plant interactions in Pseudomonas syringae pv. tomato strain DC3000.;Chatterjee A, Cui Y, Hasegawa H, Chatterjee AK;Applied and environmental microbiology 2007 Jun; 73(11):3684-94 [17400767]
TF
PsrA [Q87ZC1, view regulon]
Reported TF sp.
Pseudomonas syringae pv. tomato str. DC3000
Reported site sp.
Pseudomonas syringae pv. tomato str. DC3000
Created by
Grace Chandler
Curation notes
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Experimental Process

lacZ fusion assays showed that PsrA repressed aefR, activated rpoS, and repressed its own expression. Transcription start sites of rpoS, aefR, and psrA were identified by primer extension analysis. Visual sequence inspection of these promoters identified the putative PsrA binding sites. EMSA verified that PsrA binds to the promoters of these genes.

Transcription Factor Binding Sites


CAAACGGCAGTTTG
CAAACGTTCGTTTG
TAAACGCACGTTTG
CAAACGGCAGTTTG
CAAACGTTCGTTTG
TAAACGCACGTTTG

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
CAAACGGCAGTTTG rpoS,
... ... rpoS PSPTO_1564
Experimental technique details Beta-gal reporter assay - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Visual sequence inspection (nan) - activator not specified
CAAACGTTCGTTTG psrA,
... ... psrA nagZ PSPTO_3506
Experimental technique details Beta-gal reporter assay - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Visual sequence inspection (nan) - repressor not specified
TAAACGCACGTTTG aefR,
... ... aefR PSPTO_3548 PSPTO_3547
Experimental technique details Beta-gal reporter assay - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Visual sequence inspection (nan) - repressor not specified