Curation Information

Publication
The distal ExsA-binding site in Pseudomonas aeruginosa type III secretion system promoters is the primary determinant for promoter-specific properties.;Brutinel ED, King JM, Marsden AE, Yahr TL;Journal of bacteriology 2012 May; 194(10):2564-72 [22408165]
TF
ExsA [P26993, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Dinara Sagitova
Curation notes
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Experimental Process

Site-directed mutagenesis with lacZ fusion assays confirmed that GnC and TGnnA sequences in the exsC promoter are required for ExsA-mediated regulation. EMSA verified ExsA binding to its target promoter.

Transcription Factor Binding Sites


GATGTGGCTTTTTTCTTAA
GATGTGGCTTTTTTCTTAA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type