Curation Information

The transcription factor DNR from Pseudomonas aeruginosa specifically requires nitric oxide and haem for the activation of a target promoter in Escherichia coli.;Castiglione N, Rinaldo S, Giardina G, Cutruzzolà F;Microbiology (Reading, England) 2009 Sep; 155(Pt 9):2838-44 [19477902]
DNR [G3XCW6, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Dinara Sagitova
Curation notes

Experimental Process

Promoter-lacZ fusion assays showed DNR-mediated expression regulation under anaerobic conditions and in the presence of 10 μM NO. A canonical FNR recognition motif was identified in the nor promoter region. Specific DNR-FNR box interaction was shown by site-directed mutagenesis with lacZ reporter assays.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... norC norB PA0525
Experimental technique details Beta-gal reporter assay - Experimental technique details Site directed mutagenesis (ECO:0005667) - Experimental technique details Visual sequence inspection (nan) - Experimental technique details Western blot (quantitative) expression analysis (ECO:0000279) - activator dimer