Curation Information

Publication
Genome-wide identification of in vivo binding sites of GlxR, a cyclic AMP receptor protein-type regulator in Corynebacterium glutamicum.;Toyoda K, Teramoto H, Inui M, Yukawa H;Journal of bacteriology 2011 Aug; 193(16):4123-33 [21665967]
TF
GlxR [Q79VI7, view regulon]
Reported TF sp.
Corynebacterium glutamicum R
Reported site sp.
Corynebacterium glutamicum R
Created by
Dinara Sagitova
Curation notes
-

Experimental Process

GlxR binding sites were identified in vivo by ChIP-chip analyses. A MEME alignment of the upstream sequences of the genes with enrichment factor greater than 1 produced a GlxR binding motif. EMSAs of representative in vivo GlxR-binding promoter regions detected by ChIP-chip confirmed that GlxR binds to these promoters. Site-directed mutagenesis with EMSA showed that changing the most conserved bases abolished the GlxR binding. Each of the promoter region tested by EMSA was used in lacZ reporter assays to further validate GlxR-mediated expression.

ChIP assay conditions
Exponentially growing C. glutamicum cultures (35 ml) at an optical density at 610 nm (OD610) of up to 2.5 were treated with formaldehyde (at a final concentration of 1%) and incubated for 20 min at room temperature. The cross-linking was quenched by addition of glycine (at a final concentration of 125 mM) and incubation for 10 min at room temperature.
ChIP notes
Cells were then collected by centrifugation, washed twice with Tris-buffered saline (20 mM Tris-HCl [pH 7.5], 150 mM NaCl), and stored at −80°C. Pellets were resuspended in 2 ml IP buffer (50 mM HEPES-KOH [pH 7.5], 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.1% SDS, Roche Antiprotease mini). The cells were mechanically disrupted using a FastPrep FP120 instrument (Bio 101, Thermo Savant) as described previously (78), and the supernatant after centrifugation was sonicated on ice to shear DNA to an average size of 600 to 1,000 bp. A 50-μl fraction of the supernatant was saved for later analysis (reference DNA). The remainder was subjected to immunoprecipitation with 100 μl of magnetic beads coated with protein G (Invitrogen), which was coupled to the monoclonal anti-Strep-tag II antibody (Qiagen). The mixture was incubated overnight on a rotating platform at 4°C. The beads were washed once with IP buffer, twice with IP buffer containing 400 mM NaCl, eight times with radioimmunoprecipitation assay (RIPA) buffer (50 mM HEPES [pH 7.6], 500 mM LiCl, 1 mM EDTA, 1% NP-40, 0.7% sodium deoxycholate), and twice with Tris-EDTA (TE) buffer with 50 mM NaCl. Immunoprecipitated complexes were eluted from the beads by treatment with 210 μl elution buffer (50 mM Tris-HCl [pH 8.0], 10 mM EDTA, 1% SDS) at 65°C for 20 min. Cross-links of immunoprecipitated samples and of total DNA samples were reversed by incubation overnight at 65°C. Samples were then treated with RNase A and proteinase K for 2 h at 55°C. DNA was extracted with phenol-chloroform and purified with a QIAquick PCR purification minElute kit (Qiagen). DNA samples were blunted with T4 DNA polymerase, ligated to linkers, and amplified by PCR. Amplified DNAs from reference DNA and immunoprecipitated DNA were differentially labeled with Cy3 and Cy5, respectively, by using a CGH labeling kit (Invitrogen) according to the manufacturer's instructions. Hybridization to microarrays and array scanning were done as described previously (38). The arrays were spotted with 3,056 duplicate PCR products corresponding to C. glutamicum open reading frames (ORFs) (38). Data were normalized so that the mean of the Cy5/Cy3 intensity ratio of all of the features except the flagged ones was equal to 1, using GenePix 5.0 software. The enrichment factor for a given gene was calculated as the log2 ratio of hybridization of immunoprecipitated DNA to reference DNA. The entire procedure was carried out at least three times, and the results were averaged. A result was considered significant when the mean value was higher than 0.5 and with an associated P value of lower than 0.05.

Transcription Factor Binding Sites


TGAGATGCGCCTCACA
TGTGAGGCGCATCTCA
GGTGGCGCACTAGACA
TGTGTTCTGGGTGACA
TGCGAACAGGGGCACA
CATGATCTAAATCACA
TGTGCCCCTGTTCGCA
TGTGATTTAGATCATG
GGTGGCGTGTGTCACG
TGTGACTGTCCTCATT
AGTGTCGCAGGCCACA
AATGAGGACAGTCACA
TGTGGCCTGCGACACT
GGTGACCCAGCCCACC
GGTGGGCTGGGTCACC
TGTCCTCATGTCCACA
TGTGAGTCATGTCATT
TGTGGTTGGTGCCACA
CGTGAGGGTCGCCACA
TGCGTAGTGGATCACA
TGTGTTACACATCACA
AGTGATCGAATCCACA
CGTGAAATCGGACACA
CGTGCATGAATTCACC
AGTGGGATTCGCCACG
TGTTAAATGAGACACT
TGTGATTTCAGACACA
TGTGGGCAAGCTCACT
TGTGAGCCCACTCACC
TGTGATGCATTCGACA
CGTGATCCAAAGCACA
TGTGATCCCAGACACT
AGTGTCTGGGATCACA
AGTGGAGTCCCTCATG
AGTGGTCTTTGCCACA
TGTGGCAAATGACACT
TGTGGCAAATGACACT
TGTGGCAGCTGTCACC
TGTGACCTATAGTACA
CGTGATCCGTATCGCA
CGTGGATTCACTCCCA
TGTATCTCACCTCACA
GGTGGCACCATTCACT
TATGAATCACATCACT
AGTGATGTGATTCATA
TGTATATCTAACCACA
ATTGACCCAGATCACA
TATAACGCACTTCACA
TGTGATGCTCGCAACG
TGTGGTCAAACGCACT
AATGAAGTTTTCCACA
TGTGGAAAACTTCATT
AGTGACCTAGATCGCT
GGTGGTCGGCAGCACG
GATGAGGTTTTTCACA
AGTGTCCCGAAACACC
TGTGGCCTGTCACACA
GGTGATTTGCATCACT
AGTGGATTTAATCAAT
TGTGTCAGATCTCACC
TGTCTTCTGGAACACT
TGTGCGAAACGTCACC
TGTGCAATGCATCACG
TGTGTCTTCCACCACA
TGTGAATTACCTCACA
TGTGTTCCACCCCACA
TGTGAATTACCTCACA
TGTGTTCCACCCCACA
TGTGGGCTGGACCACG
TGTACTGTACATCACA
TGTTGACGCATCCACA
TGTGATTTATGTTACA
AGTGATCGATGCCTCA
TGTGATGCACACGACA
AGTGGACTGGTACACA
TGTGGTGAGAAACATA
TGTGATCGACAACATA
TGTGAGTTAGGTAACT
TGTGACCTACACCCCA
AGTGACCCCGCACACA
AGTGACGTGTATTACA
TGTAATACACGTCACT
TGTGAAAACTATCACA
AGTGACCCAAAGCACA
AGTGAACGGCGAAACA
TGTAATCGGCAACACT
AGTCACATAAATCACT
GATGATCTACATCACA
AGTGACGTTTGACACC
TGTGTCCTGTAGCACA
TGAGAAATAAGGCACA
TGTACCACCGCTCACA
TGTCGGATACGTCACT
GGAGAACAACGTCACC
TATGCCGAAGGCCACA
TGGGTGGTGGGTCACA
GGTGTGCAACCTCATA
GGTGATCTGCGTCAAA
AGTGACACGCGCCACC
TGGGACATTGCTCACA
AGTGTTGTAGTTCACA
GGTGGGCATCGGCACG
TGTGTCGTAATTCACT
GGTGGCTACAACCACA
TGTGGTGTGCTGCACA
CGTGGCAAAGGTCACC
TGTGCGTTATTTCACC
GGTGATGGATACGGCA
TGTGGATAACCGCACG
TGTACTGCTAATCACA
TGTGAAAGCGTACACA
TGTCTCAATCGCCACA
AGTAAACGATTACATA
CGTGGTGGCTGGCACA
AGTGACCTAAGCTACA
CGTGAGAACCATCACA
AGTGCTTAACACCACT
TGTGACTAATCACACC
TATGGCATATGTCACG
TGTATTACAGATCACA
GGTGACATGGTTCACC
TGTTCTAGGCTTCAAC
TGTTGTGTGAATCACA
TGAGATTTAGCTCACA
TGTGAGTTGCATCACA
TGTGTCGATGACCACT
TGTGGTCAGACTCACA
TGTGCCTTGCAACACA
TGTGATAGATCGCACA
GGTGAGGTTACTCACA
CGTAGTCTAGATCACA
GGTGAATATTCGCACA
AGTGACTGAGGCCACA
TGAGACCATCGTCACA
TGTGCAACTATTCAAA
AGTGTCTTGATTCACA
TATGACGCAGACCACA
TGTGCTTCGAAACTCA
TGTCTCCATTGTCACT
TGTGATTTAGGCGACA
TGTGTTTGCAGTCACC
AGTGGCAAAAGTCACT
TGGGCGGCTCATCACA
TGTGGTGGCTGGCACT
TGTGAGTCCGGTGATT
TGTGACGAGCACCCCA
TGTGACGTGGCATACA
AGTGATTTGAACCACA
AGTGTTGCTGATCACT
GGTGACTTCGCCCACG
AGTGGGGTGCTACACA
TGTGGGGGATCTCACT
AGTGACTGGAATCACC
TGTTGTACATTACACA
GGTGTTATGGGTCACT
TGTGTTTCAAATCACT
TGAGATCTATATCACT
TGTGAATCACATCACC
TGTGAGAAGAGACATA
TGTACAAGCCAACACA
TGTAAGCAATATCACC
TGTGGAATATGCCACT
TATGATGAAATTCACC
AGTGATAAGCGGCACA
TGTGACCTGCAACACA
AGTGACTCAATTCACT
AGTGACCCTACCCACA
TGTGACCATACACACC
CGTGATCTTAGTCACT
TGAGACCTGTTTCACA
TGTATGAAACCTCACT
TTTGTTTACTATCACT
AGTGATGTGTGACGCA
AGTGAGTTGAAACACT
AGTGTTAGACTTCACA
CGTGTGTCCGATCAGA
GGTTATCCAGCTCACA
TGTGGGGCTATGCACA
TGTGCAGCGCACCACA
TGTGATTGCCCACGCG
AGTGCATTAGGTCATA
TGTGATCTGAGACTCT
TGTGAGCTGAACTACA
TGTGAGGCAGCACACA
AGTGCCAGGAGGCACA
GGTGAGCTCATGCACA
CGGGAGTGGAATCAGA
TGTCACCTGATGCACA
TGTGCGCGTTGTCACA
CGTAATGTGGGTCACA
AGTGATCGAGCGCACA
AGTGTCGTGACTCACT
TGTGGTGTGGAACACT
AGTGTTCCACACCACA
AGTGGTGCAAACCACT
TGTGAGATCCACCATA
TTTGAGTCTCAACACA
TGTGGCTATCTCCACA
TGTGGTTGTCGTTACA
GGTGAGACAAGTTACA
TGTGATGTCATAAACA
TGTGTTGTGGGTTACT
GGTGATTGGTTTGTCA
TGAGATGCGCCTCACA
TGTGAGGCGCATCTCA
GGTGGCGCACTAGACA
TGTGTTCTGGGTGACA
TGCGAACAGGGGCACA
CATGATCTAAATCACA
TGTGCCCCTGTTCGCA
TGTGATTTAGATCATG
GGTGGCGTGTGTCACG
TGTGACTGTCCTCATT
AGTGTCGCAGGCCACA
AATGAGGACAGTCACA
TGTGGCCTGCGACACT
GGTGACCCAGCCCACC
GGTGGGCTGGGTCACC
TGTCCTCATGTCCACA
TGTGAGTCATGTCATT
TGTGGTTGGTGCCACA
CGTGAGGGTCGCCACA
TGCGTAGTGGATCACA
TGTGTTACACATCACA
AGTGATCGAATCCACA
CGTGAAATCGGACACA
CGTGCATGAATTCACC
AGTGGGATTCGCCACG
TGTTAAATGAGACACT
TGTGATTTCAGACACA
TGTGGGCAAGCTCACT
TGTGAGCCCACTCACC
TGTGATGCATTCGACA
CGTGATCCAAAGCACA
TGTGATCCCAGACACT
AGTGTCTGGGATCACA
AGTGGAGTCCCTCATG
AGTGGTCTTTGCCACA
TGTGGCAAATGACACT
TGTGGCAAATGACACT
TGTGGCAGCTGTCACC
TGTGACCTATAGTACA
CGTGATCCGTATCGCA
CGTGGATTCACTCCCA
TGTATCTCACCTCACA
GGTGGCACCATTCACT
TATGAATCACATCACT
AGTGATGTGATTCATA
TGTATATCTAACCACA
ATTGACCCAGATCACA
TATAACGCACTTCACA
TGTGATGCTCGCAACG
TGTGGTCAAACGCACT
AATGAAGTTTTCCACA
TGTGGAAAACTTCATT
AGTGACCTAGATCGCT
GGTGGTCGGCAGCACG
GATGAGGTTTTTCACA
AGTGTCCCGAAACACC
TGTGGCCTGTCACACA
GGTGATTTGCATCACT
AGTGGATTTAATCAAT
TGTGTCAGATCTCACC
TGTCTTCTGGAACACT
TGTGCGAAACGTCACC
TGTGCAATGCATCACG
TGTGTCTTCCACCACA
TGTGAATTACCTCACA
TGTGTTCCACCCCACA
TGTGAATTACCTCACA
TGTGTTCCACCCCACA
TGTGGGCTGGACCACG
TGTACTGTACATCACA
TGTTGACGCATCCACA
TGTGATTTATGTTACA
AGTGATCGATGCCTCA
TGTGATGCACACGACA
AGTGGACTGGTACACA
TGTGGTGAGAAACATA
TGTGATCGACAACATA
TGTGAGTTAGGTAACT
TGTGACCTACACCCCA
AGTGACCCCGCACACA
AGTGACGTGTATTACA
TGTAATACACGTCACT
TGTGAAAACTATCACA
AGTGACCCAAAGCACA
AGTGAACGGCGAAACA
TGTAATCGGCAACACT
AGTCACATAAATCACT
GATGATCTACATCACA
AGTGACGTTTGACACC
TGTGTCCTGTAGCACA
TGAGAAATAAGGCACA
TGTACCACCGCTCACA
TGTCGGATACGTCACT
GGAGAACAACGTCACC
TATGCCGAAGGCCACA
TGGGTGGTGGGTCACA
GGTGTGCAACCTCATA
GGTGATCTGCGTCAAA
AGTGACACGCGCCACC
TGGGACATTGCTCACA
AGTGTTGTAGTTCACA
GGTGGGCATCGGCACG
TGTGTCGTAATTCACT
GGTGGCTACAACCACA
TGTGGTGTGCTGCACA
CGTGGCAAAGGTCACC
TGTGCGTTATTTCACC
GGTGATGGATACGGCA
TGTGGATAACCGCACG
TGTACTGCTAATCACA
TGTGAAAGCGTACACA
TGTCTCAATCGCCACA
AGTAAACGATTACATA
CGTGGTGGCTGGCACA
AGTGACCTAAGCTACA
CGTGAGAACCATCACA
AGTGCTTAACACCACT
TGTGACTAATCACACC
TATGGCATATGTCACG
TGTATTACAGATCACA
GGTGACATGGTTCACC
TGTTCTAGGCTTCAAC
TGTTGTGTGAATCACA
TGAGATTTAGCTCACA
TGTGAGTTGCATCACA
TGTGTCGATGACCACT
TGTGGTCAGACTCACA
TGTGCCTTGCAACACA
TGTGATAGATCGCACA
GGTGAGGTTACTCACA
CGTAGTCTAGATCACA
GGTGAATATTCGCACA
AGTGACTGAGGCCACA
TGAGACCATCGTCACA
TGTGCAACTATTCAAA
AGTGTCTTGATTCACA
TATGACGCAGACCACA
TGTGCTTCGAAACTCA
TGTCTCCATTGTCACT
TGTGATTTAGGCGACA
TGTGTTTGCAGTCACC
AGTGGCAAAAGTCACT
TGGGCGGCTCATCACA
TGTGGTGGCTGGCACT
TGTGAGTCCGGTGATT
TGTGACGAGCACCCCA
TGTGACGTGGCATACA
AGTGATTTGAACCACA
AGTGTTGCTGATCACT
GGTGACTTCGCCCACG
AGTGGGGTGCTACACA
TGTGGGGGATCTCACT
AGTGACTGGAATCACC
TGTTGTACATTACACA
GGTGTTATGGGTCACT
TGTGTTTCAAATCACT
TGAGATCTATATCACT
TGTGAATCACATCACC
TGTGAGAAGAGACATA
TGTACAAGCCAACACA
TGTAAGCAATATCACC
TGTGGAATATGCCACT
TATGATGAAATTCACC
AGTGATAAGCGGCACA
TGTGACCTGCAACACA
AGTGACTCAATTCACT
AGTGACCCTACCCACA
TGTGACCATACACACC
CGTGATCTTAGTCACT
TGAGACCTGTTTCACA
TGTATGAAACCTCACT
TTTGTTTACTATCACT
AGTGATGTGTGACGCA
AGTGAGTTGAAACACT
AGTGTTAGACTTCACA
CGTGTGTCCGATCAGA
GGTTATCCAGCTCACA
TGTGGGGCTATGCACA
TGTGCAGCGCACCACA
TGTGATTGCCCACGCG
AGTGCATTAGGTCATA
TGTGATCTGAGACTCT
TGTGAGCTGAACTACA
TGTGAGGCAGCACACA
AGTGCCAGGAGGCACA
GGTGAGCTCATGCACA
CGGGAGTGGAATCAGA
TGTCACCTGATGCACA
TGTGCGCGTTGTCACA
CGTAATGTGGGTCACA
AGTGATCGAGCGCACA
AGTGTCGTGACTCACT
TGTGGTGTGGAACACT
AGTGTTCCACACCACA
AGTGGTGCAAACCACT
TGTGAGATCCACCATA
TTTGAGTCTCAACACA
TGTGGCTATCTCCACA
TGTGGTTGTCGTTACA
GGTGAGACAAGTTACA
TGTGATGTCATAAACA
TGTGTTGTGGGTTACT
GGTGATTGGTTTGTCA

Quantitative data format: Enrichment factor

TGAGATGCGCCTCACA 0.98
TGTGAGGCGCATCTCA 0.98
GGTGGCGCACTAGACA 1.33
TGTGTTCTGGGTGACA 0.93
TGCGAACAGGGGCACA 0.97
CATGATCTAAATCACA 0.97
TGTGCCCCTGTTCGCA 0.97
TGTGATTTAGATCATG 0.97
GGTGGCGTGTGTCACG 0.89
TGTGACTGTCCTCATT 2.21
AGTGTCGCAGGCCACA 2.21
AATGAGGACAGTCACA 2.21
TGTGGCCTGCGACACT 2.21
GGTGACCCAGCCCACC 1.19
GGTGGGCTGGGTCACC 1.19
TGTCCTCATGTCCACA 0.5
TGTGAGTCATGTCATT 0.73
TGTGGTTGGTGCCACA 1.54
CGTGAGGGTCGCCACA 1.17
TGCGTAGTGGATCACA 0.58
TGTGTTACACATCACA 2.17
AGTGATCGAATCCACA 0.56
CGTGAAATCGGACACA 1.13
CGTGCATGAATTCACC 1.13
AGTGGGATTCGCCACG 0.63
TGTTAAATGAGACACT 1.4
TGTGATTTCAGACACA 0.55
TGTGGGCAAGCTCACT 2.66
TGTGAGCCCACTCACC 2.66
TGTGATGCATTCGACA 1.47
CGTGATCCAAAGCACA 1.22
TGTGATCCCAGACACT 2.42
AGTGTCTGGGATCACA 2.42
AGTGGAGTCCCTCATG 0.52
AGTGGTCTTTGCCACA 1.25
TGTGGCAAATGACACT 1.18
TGTGGCAAATGACACT 1.18
TGTGGCAGCTGTCACC 0.84
TGTGACCTATAGTACA 1.01
CGTGATCCGTATCGCA 0.54
CGTGGATTCACTCCCA 0.54
TGTATCTCACCTCACA 0.82
GGTGGCACCATTCACT 0.66
TATGAATCACATCACT 1.53
AGTGATGTGATTCATA 1.53
TGTATATCTAACCACA 0.63
ATTGACCCAGATCACA 0.74
TATAACGCACTTCACA 0.5
TGTGATGCTCGCAACG 0.55
TGTGGTCAAACGCACT 0.69
AATGAAGTTTTCCACA 1.31
TGTGGAAAACTTCATT 1.31
AGTGACCTAGATCGCT 0.73
GGTGGTCGGCAGCACG 0.58
GATGAGGTTTTTCACA 0.5
AGTGTCCCGAAACACC 0.86
TGTGGCCTGTCACACA 0.62
GGTGATTTGCATCACT 0.54
AGTGGATTTAATCAAT 0.91
TGTGTCAGATCTCACC 0.66
TGTCTTCTGGAACACT 0.66
TGTGCGAAACGTCACC 1.42
TGTGCAATGCATCACG 2.46
TGTGTCTTCCACCACA 2.21
TGTGAATTACCTCACA 1.36
TGTGTTCCACCCCACA 1.36
TGTGAATTACCTCACA 1.36
TGTGTTCCACCCCACA 1.36
TGTGGGCTGGACCACG 0.89
TGTACTGTACATCACA 1.26
TGTTGACGCATCCACA 1.26
TGTGATTTATGTTACA 1.51
AGTGATCGATGCCTCA 1.79
TGTGATGCACACGACA 0.7
AGTGGACTGGTACACA 0.7
TGTGGTGAGAAACATA 0.67
TGTGATCGACAACATA 0.67
TGTGAGTTAGGTAACT 0.67
TGTGACCTACACCCCA 1.07
AGTGACCCCGCACACA 1.27
AGTGACGTGTATTACA 0.77
TGTAATACACGTCACT 0.77
TGTGAAAACTATCACA 0.83
AGTGACCCAAAGCACA 1.57
AGTGAACGGCGAAACA 1.57
TGTAATCGGCAACACT 0.73
AGTCACATAAATCACT 0.57
GATGATCTACATCACA 0.93
AGTGACGTTTGACACC 0.86
TGTGTCCTGTAGCACA 2.51
TGAGAAATAAGGCACA 0.64
TGTACCACCGCTCACA 0.64
TGTCGGATACGTCACT 1.06
GGAGAACAACGTCACC 0.92
TATGCCGAAGGCCACA 0.92
TGGGTGGTGGGTCACA 1.04
GGTGTGCAACCTCATA 1.04
GGTGATCTGCGTCAAA 0.67
AGTGACACGCGCCACC 0.67
TGGGACATTGCTCACA 0.67
AGTGTTGTAGTTCACA 1.61
GGTGGGCATCGGCACG 0.69
TGTGTCGTAATTCACT 1.37
GGTGGCTACAACCACA 0.51
TGTGGTGTGCTGCACA 2.36
CGTGGCAAAGGTCACC 0.82
TGTGCGTTATTTCACC 0.54
GGTGATGGATACGGCA 0.63
TGTGGATAACCGCACG 0.92
TGTACTGCTAATCACA 0.92
TGTGAAAGCGTACACA 1.08
TGTCTCAATCGCCACA 0.76
AGTAAACGATTACATA 0.76
CGTGGTGGCTGGCACA 1.05
AGTGACCTAAGCTACA 0.53
CGTGAGAACCATCACA 1.59
AGTGCTTAACACCACT 1.59
TGTGACTAATCACACC 0.69
TATGGCATATGTCACG 0.63
TGTATTACAGATCACA 1.54
GGTGACATGGTTCACC 0.9
TGTTCTAGGCTTCAAC 0.92
TGTTGTGTGAATCACA 0.81
TGAGATTTAGCTCACA 0.7
TGTGAGTTGCATCACA 0.77
TGTGTCGATGACCACT 1.08
TGTGGTCAGACTCACA 0.56
TGTGCCTTGCAACACA 1.4
TGTGATAGATCGCACA 1.62
GGTGAGGTTACTCACA 0.52
CGTAGTCTAGATCACA 0.62
GGTGAATATTCGCACA 0.62
AGTGACTGAGGCCACA 1.36
TGAGACCATCGTCACA 1.36
TGTGCAACTATTCAAA 1.36
AGTGTCTTGATTCACA 1.36
TATGACGCAGACCACA 1.12
TGTGCTTCGAAACTCA 0.87
TGTCTCCATTGTCACT 0.87
TGTGATTTAGGCGACA 0.9
TGTGTTTGCAGTCACC 0.53
AGTGGCAAAAGTCACT 2.43
TGGGCGGCTCATCACA 0.55
TGTGGTGGCTGGCACT 1.85
TGTGAGTCCGGTGATT 0.78
TGTGACGAGCACCCCA 0.58
TGTGACGTGGCATACA 0.81
AGTGATTTGAACCACA 1.82
AGTGTTGCTGATCACT 2.14
GGTGACTTCGCCCACG 2.14
AGTGGGGTGCTACACA 0.75
TGTGGGGGATCTCACT 1.36
AGTGACTGGAATCACC 0.78
TGTTGTACATTACACA 0.83
GGTGTTATGGGTCACT 0.74
TGTGTTTCAAATCACT 2.07
TGAGATCTATATCACT 0.95
TGTGAATCACATCACC 0.81
TGTGAGAAGAGACATA 0.77
TGTACAAGCCAACACA 0.77
TGTAAGCAATATCACC 1.04
TGTGGAATATGCCACT 1.04
TATGATGAAATTCACC 0.56
AGTGATAAGCGGCACA 0.96
TGTGACCTGCAACACA 1.81
AGTGACTCAATTCACT 0.52
AGTGACCCTACCCACA 0.65
TGTGACCATACACACC 0.52
CGTGATCTTAGTCACT 0.9
TGAGACCTGTTTCACA 0.9
TGTATGAAACCTCACT 1.03
TTTGTTTACTATCACT 0.69
AGTGATGTGTGACGCA 0.69
AGTGAGTTGAAACACT 1.48
AGTGTTAGACTTCACA 1.48
CGTGTGTCCGATCAGA 0.58
GGTTATCCAGCTCACA 0.78
TGTGGGGCTATGCACA 1.28
TGTGCAGCGCACCACA 1.25
TGTGATTGCCCACGCG 0.66
AGTGCATTAGGTCATA 0.94
TGTGATCTGAGACTCT 1.38
TGTGAGCTGAACTACA 0.72
TGTGAGGCAGCACACA 1.95
AGTGCCAGGAGGCACA 0.86
GGTGAGCTCATGCACA 0.59
CGGGAGTGGAATCAGA 0.96
TGTCACCTGATGCACA 1.68
TGTGCGCGTTGTCACA 1.68
CGTAATGTGGGTCACA 1.02
AGTGATCGAGCGCACA 0.51
AGTGTCGTGACTCACT 1.96
TGTGGTGTGGAACACT 0.61
AGTGTTCCACACCACA 0.61
AGTGGTGCAAACCACT 1.19
TGTGAGATCCACCATA 0.76
TTTGAGTCTCAACACA 0.76
TGTGGCTATCTCCACA 0.54
TGTGGTTGTCGTTACA 1.13
GGTGAGACAAGTTACA 0.53
TGTGATGTCATAAACA 0.53
TGTGTTGTGGGTTACT 0.92
GGTGATTGGTTTGTCA 0.87
TGAGATGCGCCTCACA 0.98
TGTGAGGCGCATCTCA 1.6
GGTGGCGCACTAGACA 1.33
TGTGTTCTGGGTGACA 0.93
TGCGAACAGGGGCACA 0.97
CATGATCTAAATCACA 0.97
TGTGCCCCTGTTCGCA 1.53
TGTGATTTAGATCATG 1.53
GGTGGCGTGTGTCACG 0.89
TGTGACTGTCCTCATT 2.21
AGTGTCGCAGGCCACA 2.21
AATGAGGACAGTCACA 1.02
TGTGGCCTGCGACACT 1.02
GGTGACCCAGCCCACC 1.19
GGTGGGCTGGGTCACC 0.5
TGTCCTCATGTCCACA 0.5
TGTGAGTCATGTCATT 0.73
TGTGGTTGGTGCCACA 1.54
CGTGAGGGTCGCCACA 1.17
TGCGTAGTGGATCACA 0.58
TGTGTTACACATCACA 2.17
AGTGATCGAATCCACA 0.56
CGTGAAATCGGACACA 1.13
CGTGCATGAATTCACC 1.13
AGTGGGATTCGCCACG 0.63
TGTTAAATGAGACACT 1.4
TGTGATTTCAGACACA 0.55
TGTGGGCAAGCTCACT 2.66
TGTGAGCCCACTCACC 2.66
TGTGATGCATTCGACA 1.47
CGTGATCCAAAGCACA 1.22
TGTGATCCCAGACACT 2.42
AGTGTCTGGGATCACA 1.43
AGTGGAGTCCCTCATG 0.52
AGTGGTCTTTGCCACA 1.25
TGTGGCAAATGACACT 1.18
TGTGGCAAATGACACT 0.54
TGTGGCAGCTGTCACC 0.84
TGTGACCTATAGTACA 1.01
CGTGATCCGTATCGCA 0.54
CGTGGATTCACTCCCA 0.54
TGTATCTCACCTCACA 0.82
GGTGGCACCATTCACT 0.66
TATGAATCACATCACT 1.53
AGTGATGTGATTCATA 0.98
TGTATATCTAACCACA 0.63
ATTGACCCAGATCACA 0.74
TATAACGCACTTCACA 0.5
TGTGATGCTCGCAACG 0.55
TGTGGTCAAACGCACT 0.69
AATGAAGTTTTCCACA 1.31
TGTGGAAAACTTCATT 1.12
AGTGACCTAGATCGCT 0.73
GGTGGTCGGCAGCACG 0.58
GATGAGGTTTTTCACA 0.5
AGTGTCCCGAAACACC 0.86
TGTGGCCTGTCACACA 0.62
GGTGATTTGCATCACT 0.54
AGTGGATTTAATCAAT 0.91
TGTGTCAGATCTCACC 0.66
TGTCTTCTGGAACACT 0.66
TGTGCGAAACGTCACC 1.42
TGTGCAATGCATCACG 2.46
TGTGTCTTCCACCACA 2.21
TGTGAATTACCTCACA 1.36
TGTGTTCCACCCCACA 1.36
TGTGAATTACCTCACA 1.41
TGTGTTCCACCCCACA 1.41
TGTGGGCTGGACCACG 0.89
TGTACTGTACATCACA 1.26
TGTTGACGCATCCACA 1.26
TGTGATTTATGTTACA 1.51
AGTGATCGATGCCTCA 1.79
TGTGATGCACACGACA 0.7
AGTGGACTGGTACACA 0.7
TGTGGTGAGAAACATA 0.67
TGTGATCGACAACATA 0.67
TGTGAGTTAGGTAACT 0.67
TGTGACCTACACCCCA 1.07
AGTGACCCCGCACACA 1.27
AGTGACGTGTATTACA 0.77
TGTAATACACGTCACT 0.85
TGTGAAAACTATCACA 0.83
AGTGACCCAAAGCACA 1.57
AGTGAACGGCGAAACA 1.57
TGTAATCGGCAACACT 0.73
AGTCACATAAATCACT 0.57
GATGATCTACATCACA 0.93
AGTGACGTTTGACACC 0.86
TGTGTCCTGTAGCACA 2.51
TGAGAAATAAGGCACA 0.64
TGTACCACCGCTCACA 0.64
TGTCGGATACGTCACT 1.06
GGAGAACAACGTCACC 0.92
TATGCCGAAGGCCACA 0.92
TGGGTGGTGGGTCACA 1.04
GGTGTGCAACCTCATA 1.04
GGTGATCTGCGTCAAA 0.67
AGTGACACGCGCCACC 0.67
TGGGACATTGCTCACA 0.67
AGTGTTGTAGTTCACA 1.61
GGTGGGCATCGGCACG 0.69
TGTGTCGTAATTCACT 1.37
GGTGGCTACAACCACA 0.51
TGTGGTGTGCTGCACA 2.36
CGTGGCAAAGGTCACC 0.82
TGTGCGTTATTTCACC 0.54
GGTGATGGATACGGCA 0.63
TGTGGATAACCGCACG 0.92
TGTACTGCTAATCACA 0.92
TGTGAAAGCGTACACA 1.08
TGTCTCAATCGCCACA 0.76
AGTAAACGATTACATA 0.76
CGTGGTGGCTGGCACA 1.05
AGTGACCTAAGCTACA 0.53
CGTGAGAACCATCACA 1.59
AGTGCTTAACACCACT 1.59
TGTGACTAATCACACC 0.69
TATGGCATATGTCACG 0.63
TGTATTACAGATCACA 1.54
GGTGACATGGTTCACC 0.9
TGTTCTAGGCTTCAAC 0.92
TGTTGTGTGAATCACA 0.81
TGAGATTTAGCTCACA 0.7
TGTGAGTTGCATCACA 0.77
TGTGTCGATGACCACT 1.08
TGTGGTCAGACTCACA 0.56
TGTGCCTTGCAACACA 1.4
TGTGATAGATCGCACA 1.62
GGTGAGGTTACTCACA 0.52
CGTAGTCTAGATCACA 0.62
GGTGAATATTCGCACA 0.62
AGTGACTGAGGCCACA 1.36
TGAGACCATCGTCACA 1.36
TGTGCAACTATTCAAA 1.36
AGTGTCTTGATTCACA 1.36
TATGACGCAGACCACA 1.12
TGTGCTTCGAAACTCA 0.87
TGTCTCCATTGTCACT 0.87
TGTGATTTAGGCGACA 0.9
TGTGTTTGCAGTCACC 0.53
AGTGGCAAAAGTCACT 2.43
TGGGCGGCTCATCACA 0.55
TGTGGTGGCTGGCACT 1.85
TGTGAGTCCGGTGATT 0.78
TGTGACGAGCACCCCA 0.58
TGTGACGTGGCATACA 0.81
AGTGATTTGAACCACA 1.82
AGTGTTGCTGATCACT 2.14
GGTGACTTCGCCCACG 2.14
AGTGGGGTGCTACACA 0.75
TGTGGGGGATCTCACT 1.36
AGTGACTGGAATCACC 0.78
TGTTGTACATTACACA 0.83
GGTGTTATGGGTCACT 0.74
TGTGTTTCAAATCACT 2.07
TGAGATCTATATCACT 0.95
TGTGAATCACATCACC 0.81
TGTGAGAAGAGACATA 0.77
TGTACAAGCCAACACA 0.77
TGTAAGCAATATCACC 1.04
TGTGGAATATGCCACT 1.04
TATGATGAAATTCACC 0.56
AGTGATAAGCGGCACA 0.96
TGTGACCTGCAACACA 1.81
AGTGACTCAATTCACT 0.52
AGTGACCCTACCCACA 0.65
TGTGACCATACACACC 0.52
CGTGATCTTAGTCACT 0.9
TGAGACCTGTTTCACA 0.9
TGTATGAAACCTCACT 1.03
TTTGTTTACTATCACT 0.69
AGTGATGTGTGACGCA 0.69
AGTGAGTTGAAACACT 1.48
AGTGTTAGACTTCACA 1.48
CGTGTGTCCGATCAGA 0.58
GGTTATCCAGCTCACA 0.78
TGTGGGGCTATGCACA 1.28
TGTGCAGCGCACCACA 1.25
TGTGATTGCCCACGCG 0.66
AGTGCATTAGGTCATA 0.94
TGTGATCTGAGACTCT 1.38
TGTGAGCTGAACTACA 0.72
TGTGAGGCAGCACACA 1.95
AGTGCCAGGAGGCACA 0.86
GGTGAGCTCATGCACA 0.59
CGGGAGTGGAATCAGA 0.96
TGTCACCTGATGCACA 1.68
TGTGCGCGTTGTCACA 1.68
CGTAATGTGGGTCACA 1.02
AGTGATCGAGCGCACA 0.51
AGTGTCGTGACTCACT 1.96
TGTGGTGTGGAACACT 0.61
AGTGTTCCACACCACA 0.51
AGTGGTGCAAACCACT 1.19
TGTGAGATCCACCATA 0.76
TTTGAGTCTCAACACA 0.76
TGTGGCTATCTCCACA 0.54
TGTGGTTGTCGTTACA 1.13
GGTGAGACAAGTTACA 0.53
TGTGATGTCATAAACA 0.53
TGTGTTGTGGGTTACT 0.92
GGTGATTGGTTTGTCA 0.87

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TGAGATGCGCCTCACA
... ... cgR_0017 cgR_0018
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGAGGCGCATCTCA
... ... cgR_0017 cgR_0018
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
GGTGGCGCACTAGACA
... ... cgR_0033
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGTTCTGGGTGACA
... ... cgR_0046
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGCGAACAGGGGCACA
... ... cgR_0064 cgR_0063 cgR_0062 cgR_0061 cgR_0065
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
CATGATCTAAATCACA
... ... cgR_0064 cgR_0063 cgR_0062 cgR_0061 cgR_0065
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGCCCCTGTTCGCA
... ... cgR_0064 cgR_0063 cgR_0062 cgR_0061 cgR_0065
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGATTTAGATCATG
... ... cgR_0064 cgR_0063 cgR_0062 cgR_0061 cgR_0065
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
GGTGGCGTGTGTCACG
... ... cgR_0087 cgR_0086 cgR_0088 cgR_0089
Experimental technique details Beta-gal reporter assay - Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details Site directed mutagenesis (ECO:0005667) - not specified not specified
TGTGACTGTCCTCATT
... ... cgR_0115 cgR_0116 cgR_0117
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
AGTGTCGCAGGCCACA
... ... cgR_0115 cgR_0114 cgR_0116 cgR_0117
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
AATGAGGACAGTCACA
... ... cgR_0115 cgR_0116 cgR_0117
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGGCCTGCGACACT
... ... cgR_0115 cgR_0114 cgR_0116 cgR_0117
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
GGTGACCCAGCCCACC
... ... cgR_0121 cgR_0120 cgR_0122 cgR_0123 cgR_0124 cgR_0125 cgR_0126
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
GGTGGGCTGGGTCACC
... ... cgR_0121 cgR_0120 cgR_0122 cgR_0123 cgR_0124 cgR_0125 cgR_0126
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTCCTCATGTCCACA
... ... cgR_0130
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGAGTCATGTCATT
... ... cgR_0206 cgR_0207 cgR_0208 cgR_0209 cgR_0210
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGGTTGGTGCCACA
... ... cgR_0233 cgR_0234 cgR_0235
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
CGTGAGGGTCGCCACA
... ... cgR_0236 cgR_0237 cgR_0238 cgR_0239 cgR_0240 cgR_0241 cgR_0242 cgR_0243 cgR_0244 cgR_0245 cgR_0246 cgR_0247 cgR_0248
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGCGTAGTGGATCACA
... ... cgR_0249 cgR_0250 cgR_0251
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGTTACACATCACA
... ... cgR_0262 cgR_0263 gltD
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
AGTGATCGAATCCACA
... ... cgR_0273 cgR_0274 cgR_0275 cgR_0276
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
CGTGAAATCGGACACA
... ... cgR_0280 cgR_0279 cgR_0281
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
CGTGCATGAATTCACC
... ... cgR_0280 cgR_0279 cgR_0281
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
AGTGGGATTCGCCACG
... ... cgR_0291 moaC cgR_0289 cgR_0288 cgR_0287
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTTAAATGAGACACT
... ... cgR_0300 cgR_0301 cgR_0302 cgR_0303 cgR_t11 cgR_0304 cgR_0305 cgR_0306
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGATTTCAGACACA
... ... cgR_0323 cgR_0324 cgR_0325
Experimental technique details Beta-gal reporter assay - Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details Site directed mutagenesis (ECO:0005667) - not specified not specified
TGTGGGCAAGCTCACT
... ... cgR_0350 cgR_0349 cgR_0351
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGAGCCCACTCACC
... ... cgR_0350 cgR_0349 cgR_0351
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGATGCATTCGACA
... ... cgR_0365 cgR_0366 cgR_0367 cgR_0368
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
CGTGATCCAAAGCACA
... ... cgR_0370 cgR_0369
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGATCCCAGACACT
... ... cgR_0371 cgR_0372 cgR_0373 cgR_0374 cgR_0375 cgR_0376
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
AGTGTCTGGGATCACA
... ... cgR_0371 cgR_0372 cgR_0373 cgR_0374 cgR_0375 cgR_0376
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
AGTGGAGTCCCTCATG
... ... cgR_0376
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
AGTGGTCTTTGCCACA
... ... cgR_0377
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGGCAAATGACACT
... ... cgR_0385 cgR_0384 cgR_0383 cgR_0386 cgR_0387 cgR_0388 cgR_0389 cgR_0390 cgR_0391 cgR_0392
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGGCAAATGACACT
... ... cgR_0385 cgR_0384 cgR_0383 cgR_0386 cgR_0387 cgR_0388 cgR_0389 cgR_0390 cgR_0391 cgR_0392
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGGCAGCTGTCACC
... ... cgR_0445 cgR_0446
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGACCTATAGTACA
... ... cgR_0491 cgR_0492 cgR_0493 cgR_0494 cgR_0495
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
CGTGATCCGTATCGCA
... ... cgR_0513 cgR_0514 cgR_0515 cgR_0516 cgR_0517
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
CGTGGATTCACTCCCA
... ... cgR_0575 cgR_0576 cgR_t15
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTATCTCACCTCACA
... ... cgR_0581 cgR_0582 cgR_0583 cgR_0584 cgR_0585 cgR_0586 cgR_0587
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
GGTGGCACCATTCACT
... ... cgR_0586 cgR_0587
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TATGAATCACATCACT
... ... cgR_0603 cgR_0604
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
AGTGATGTGATTCATA
... ... cgR_0603 cgR_0604
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTATATCTAACCACA
... ... cgR_0619 cgR_0618 rplN rplX rplE cgR_0623
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
ATTGACCCAGATCACA
... ... paaA paaB cgR_0649 cgR_0650 cgR_0651 cgR_0652 cgR_0653 cgR_0654 cgR_0655
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TATAACGCACTTCACA
... ... cgR_0656 cgR_0657
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGATGCTCGCAACG
... ... cgR_0697 cgR_0698
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGGTCAAACGCACT
... ... cgR_0742 cgR_0741
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
AATGAAGTTTTCCACA
... ... cgR_0769 cgR_0770 cgR_0771
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGGAAAACTTCATT
... ... cgR_0769 cgR_0770 cgR_0771
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
AGTGACCTAGATCGCT
... ... cgR_0777 cgR_0778 cgR_0779
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
GGTGGTCGGCAGCACG
... ... cgR_0785 cgR_0784 cgR_0783 cgR_0782 cgR_0781 cgR_0780
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
GATGAGGTTTTTCACA
... ... cgR_0786 cgR_0785 cgR_0784 cgR_0783 cgR_0782 cgR_0781 cgR_0780
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
AGTGTCCCGAAACACC
... ... cgR_0805
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGGCCTGTCACACA
... ... cgR_0814 cgR_0815 cgR_0816
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
GGTGATTTGCATCACT
... ... cgR_0828 cgR_0829 cgR_0830 cgR_0831 cgR_0832 cgR_0833 cgR_0834 cgR_0835 cgR_0836
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
AGTGGATTTAATCAAT
... ... cgR_0847 cgR_0846 cgR_0845 cgR_0844 cgR_0843 cgR_0842
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGTCAGATCTCACC
... ... cgR_0849 cgR_0848 cgR_0850
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTCTTCTGGAACACT
... ... cgR_0849 cgR_0848 cgR_0850
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGCGAAACGTCACC
... ... cgR_0850 cgR_0849 cgR_0848 cgR_0851
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGCAATGCATCACG
... ... cgR_0866 cgR_0867 secA
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGTCTTCCACCACA
... ... cgR_0875 cgR_0876
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGAATTACCTCACA
... ... cgR_0877 cgR_0878
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGTTCCACCCCACA
... ... cgR_0877 cgR_0878
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGAATTACCTCACA
... ... cgR_0877 cgR_0878
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGTTCCACCCCACA
... ... cgR_0877 cgR_0878
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGGGCTGGACCACG
... ... cgR_0893
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTACTGTACATCACA
... ... cgR_0916 cgR_0915 cgR_0914 cgR_0913 cgR_0917
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTTGACGCATCCACA
... ... cgR_0916 cgR_0915 cgR_0914 cgR_0913 cgR_0917
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGATTTATGTTACA
... ... cgR_0934
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
AGTGATCGATGCCTCA
... ... cgR_0936 cgR_0935
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGATGCACACGACA
... ... cgR_0942 cgR_0941 cgR_0940 gltA cgR_0944
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
AGTGGACTGGTACACA
... ... cgR_0942 cgR_0941 cgR_0940 gltA cgR_0944
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGGTGAGAAACATA
... ... cgR_0945 cgR_0946 cgR_0947 cgR_0948
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGATCGACAACATA
... ... cgR_0945 cgR_0946 cgR_0947 cgR_0948
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGAGTTAGGTAACT
... ... cgR_0945 cgR_0946 cgR_0947 cgR_0948
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGACCTACACCCCA
... ... cgR_0954
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
AGTGACCCCGCACACA
... ... cgR_1015 cgR_1016 cgR_1017 cgR_1018
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
AGTGACGTGTATTACA
... ... cgR_1030 cgR_1031 cgR_1032 cgR_1033 cgR_1034 cgR_1035
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTAATACACGTCACT
... ... cgR_1030 cgR_1031 cgR_1032 cgR_1033 cgR_1034 cgR_1035
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGAAAACTATCACA
... ... cgR_1053 cgR_1054 cgR_1055
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
AGTGACCCAAAGCACA
... ... cgR_1056 cgR_1057
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
AGTGAACGGCGAAACA
... ... cgR_1056 cgR_1057
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTAATCGGCAACACT
... ... cgR_t23 cgR_1058 cgR_1059 cgR_1060
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
AGTCACATAAATCACT
... ... cgR_1068
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
GATGATCTACATCACA
... ... cgR_1069 cgR_1068 cgR_1070
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
AGTGACGTTTGACACC
... ... cgR_1094 cgR_1093
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGTCCTGTAGCACA
... ... cgR_1096 cgR_1095 cgR_1094 cgR_1093 cgR_1097 cgR_1098
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGAGAAATAAGGCACA
... ... fumC cgR_1100 cgR_5015
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTACCACCGCTCACA
... ... cgR_1100 fumC cgR_5015
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTCGGATACGTCACT
... ... cgR_1104 glpX
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
GGAGAACAACGTCACC
... ... cgR_1178 cgR_1177 cgR_1176 cgR_1175
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TATGCCGAAGGCCACA
... ... cgR_1178 cgR_1177 cgR_1176 cgR_1175
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGGGTGGTGGGTCACA
... ... cgR_1223 cgR_1224
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
GGTGTGCAACCTCATA
... ... cgR_1223 cgR_1224
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
GGTGATCTGCGTCAAA
... ... cgR_1237 cgR_1238
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
AGTGACACGCGCCACC
... ... cgR_1237 cgR_1238
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGGGACATTGCTCACA
... ... cgR_1237 cgR_1238
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
AGTGTTGTAGTTCACA
... ... cgR_1245 cgR_1244 cgR_1243 cgR_1246 cgR_1247 cgR_1248 cgR_1249
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
GGTGGGCATCGGCACG
... ... cgR_1266 cgR_1265
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGTCGTAATTCACT
... ... cgR_1269 cgR_1268 cgR_1267 cgR_1266 cgR_1265 cgR_1270
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
GGTGGCTACAACCACA
... ... cgR_1289 cgR_1290 atpC cgR_1292 cgR_1293 cgR_1294
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGGTGTGCTGCACA
... ... cgR_1326 cgR_1327
Experimental technique details Beta-gal reporter assay - Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details Site directed mutagenesis (ECO:0005667) - not specified not specified
CGTGGCAAAGGTCACC
... ... gltX cgR_1370
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGCGTTATTTCACC
... ... cgR_1385
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
GGTGATGGATACGGCA
... ... cgR_1387 cgR_1386 cgR_1385 cgR_1388
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGGATAACCGCACG
... ... cgR_1415 cgR_1414
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTACTGCTAATCACA
... ... cgR_1415
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGAAAGCGTACACA
... ... cgR_1416 cgR_1415
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTCTCAATCGCCACA
... ... cgR_1432 cgR_1431 cgR_1430 cgR_1429 cgR_1433 cgR_1434
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
AGTAAACGATTACATA
... ... cgR_1432 cgR_1431 cgR_1430 cgR_1429 cgR_1433 cgR_1434
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
CGTGGTGGCTGGCACA
... ... cgR_1477 pyrG cgR_1479 xerD
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
AGTGACCTAAGCTACA
... ... cgR_1483
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
CGTGAGAACCATCACA
... ... cgR_1484 cgR_1485 cgR_1486 cgR_1487 cmk engA
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
AGTGCTTAACACCACT
... ... cgR_1484 cgR_1485 cgR_1486 cgR_1487 cmk engA
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGACTAATCACACC
... ... cgR_1504
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TATGGCATATGTCACG
... ... cgR_1526 cgR_1527
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTATTACAGATCACA
... ... cgR_1535
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
GGTGACATGGTTCACC
... ... cgR_1569 cgR_1570 cgR_1571 cgR_1572
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTTCTAGGCTTCAAC
... ... cgR_1581 cgR_1580
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTTGTGTGAATCACA
... ... cgR_1608 cgR_1607 cgR_1609
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGAGATTTAGCTCACA
... ... cgR_1616 cgR_1615 cgR_1614 cgR_1613 cgR_1612 cgR_1611 cgR_1610 cgR_1617 cgR_1618 cgR_1619 cgR_1620 cgR_1621
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details Motif-discovery (ECO:0005558) - not specified not specified
TGTGAGTTGCATCACA
... ... cgR_1636
Experimental technique details Beta-gal reporter assay - Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details Site directed mutagenesis (ECO:0005667) - not specified not specified
TGTGTCGATGACCACT
... ... gmk cgR_1654
Experimental technique details ChIP-chip (ECO:0006007)