Transcription start sites were mapped by primer extension. Palindromic lux-box-like sites were identified by visual inspection of the promoters. Site-directed mutagenesis with lacZ reporter assays showed that changing the conserved C to T at position 3 resulted in decreased activation. Deletion of portions of the lux-box-like elements resulted in a severe decrease in signal-activated transcription
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|