Deletional analyses of the atzR and the atzDEF promoters with lacZ promoter fusions were used to identify AtzR binding sites. EMSA verified AtzR binding to both promoters. DNase I footprinting was done to localize the sequences protected by AtzR. The authors identify a repressor site consisting of two hexamers (RBS) and a secondary site consisting of three additional hexamers (ABS), but show that only RBS is strictly necessary for binding.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|