Promoter-lacZ fusion assays showed that ColR regulates the expression of PP0903. DNase I footprinting was used to identify ColR binding sites in oprQ and PP0903. ColR binding motif was then used to identify additional ColR targets. LacZ reporter assays, DNase I footprinting, EMSA were used to verify the functionality of the predicted ColR binding sites.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|