DNA microarrays identified genes differentially expressed in the CosR-mutant strain. CosR-dependent regulation of some of these genes was further verified by qRT-PCR. cmeABC-lacZ fusion assay showed that CosR represses cmeABC expression. By measuring the catalase activity, katA expression was shown to be activated by CosR. EMSA confirmed that katA promoter was bound by CosR. DNase I footprinting localized the CosR binding sites in the katA and cmeABC promoters. Alignment with the known CosR binding sites identified a new consensus (ttTaAanaAAAaTTAagaTTT).
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|
|TATTAACCAAAATTAAGATAT||cmeA, cmeB, cmeC||