Curation Information

Sinorhizobium meliloti fur-like (Mur) protein binds a fur box-like sequence present in the mntA promoter in a manganese-responsive manner.;Platero R, de Lorenzo V, Garat B, Fabiano E;Applied and environmental microbiology 2007 Aug; 73(15):4832-8 [17557847]
Mur [Q92LL6, view regulon]
Reported TF sp.
Sinorhizobium meliloti 1021
Reported site sp.
Sinorhizobium meliloti 1021
Created by
Grace Chandler
Curation notes

Experimental Process

DNase I footprinting showed that manganese-activated Mur binds to the predicted Mur binding site in the mntA promoter. EMSA analysis verified manganese-dependent binding of Mur to mntA since the affinity of Mur to its target site was 60-fold lower in the absence of manganese.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... fur sitA sitB sitC sitD
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - not specified dimer