Curation Information

Publication
Specific binding of the Xanthomonas campestris pv. vesicatoria AraC-type transcriptional activator HrpX to plant-inducible promoter boxes.;Koebnik R, Kr├╝ger A, Thieme F, Urban A, Bonas U;Journal of bacteriology 2006 Nov; 188(21):7652-60 [16936021]
TF
CmeR [Q0PBE2, view regulon]
Reported TF sp.
Campylobacter jejuni subsp. jejuni 81-176
Reported site sp.
Campylobacter jejuni subsp. jejuni 81-176
Created by
Dinara Sagitova
Curation notes
-

Experimental Process

EMSA confirmed that CmeR specifically binds to the promoter region of cmeABC. Specific CmeR binding was verified by performing a gel mobility shift assay with a series of PCR products whose sequences span different portions of the region between cmeR and cmeABC. Site-directed mutagenesis in conjunction with EMSA and lacZ reporter fusion assay showed that the single deletional mutation increased the level of transcription of cmeABC.

Transcription Factor Binding Sites


TGTAATAAATATTACA
TGTAATAAATATTACA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TGTAATAAATATTACA cmeA, cmeB, cmeC
... ... cmeA cmeB cmeC
Experimental technique details Beta-gal reporter assay - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Visual sequence inspection (nan) - repressor not specified