EMSA confirmed that CmeR specifically binds to the promoter region of cmeABC. Specific CmeR binding was verified by performing a gel mobility shift assay with a series of PCR products whose sequences span different portions of the region between cmeR and cmeABC. Site-directed mutagenesis in conjunction with EMSA and lacZ reporter fusion assay showed that the single deletional mutation increased the level of transcription of cmeABC.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|
|TGTAATAAATATTACA||cmeA, cmeB, cmeC||