Curation Information

Transcriptional analysis and functional characterization of XCC1294 gene encoding a GGDEF domain protein in Xanthomonas campestris pv. campestris.;Hsiao YM, Song WL, Liao CT, Lin IH, Pan MY, Lin CF;Archives of microbiology 2012 Apr; 194(4):293-304 [22002465]
Clp [Q4UZF6, view regulon]
Reported TF sp.
Xanthomonas campestris pv. campestris str.17
Reported site sp.
Xanthomonas campestris pv. campestris str.17
Created by
Dinara Sagitova
Curation notes

Experimental Process

Promoter-β-galactosidase reporter assays and RT-PCR experiments showed the involvement of Clp in the expressions of the GGDEF domain proteins. The XCC1294 transcription initiation point was located by the 5′ RACE method.A predicted Clp binding site, with 7/10 bases matching the consensus sequence, was located at −35/−50 relative to the XCC1294 transcription initiation point. A gel retardation assay confirmed that Clp upregulates XCC1294 expression in a direct manner.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... XCC1294 XCC1293 XCC1292
Experimental technique details Beta-gal reporter assay - Experimental technique details EMSA (ECO:0001807) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - Experimental technique details Visual sequence inspection (nan) - activator not specified