GFP promoter fusion assay, confirmed by beta-galactosidase reporter assay, indicated regulation of promoters in response to hypoxia. Transcription start points determined by primer extension. Site-directed mutagenesis inactivated suspected binding sites and expression assays confirmed reduced expression in response to hypoxia. DevR binding sites confirmed via DNAse I footprinting; 3 sites found to bind.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|