the transcriptional start site of the katA–ankA operon was mapped by primer extension analysis. Northern blot hybridization using an oxyR mutant showed that oxyR was required to mediate oxidant-dependent induction of katA expression. The direct activation of katA transcription by OxyR was shown by EMSAs. A putative OxyR binding site similar to the E.coli consensus OxyR binding motif was identified in the X. campestris pv. phaseoli katA promoter.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|