Purified Arc was used to perform EMSAs on a synthesized arc operator sequence. Wild-type and insertion-bearing (yet functional) Arc proteins were used and the difference in retardation fragments was used to determine the tetrameric nature of Arc binding (as a dimer of dimers).
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|