To identify the genes that constitute the regulon of L. lactis CodY, the transcriptional profile of L. lactis MG1363 was compared with that of L. lactis MG1363ΔcodY. β-Galactosidase assays were used to confirm the DNA microarray results. EMSAs were performed to confirm that CodY binds its target promoters.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|