Curation Information

Publication
Regulation of the pehA gene encoding the major polygalacturonase of Xanthomonas campestris by Clp and RpfF.;Hsiao YM, Zheng MH, Hu RM, Yang TC, Tseng YH;Microbiology (Reading, England) 2008 Mar; 154(Pt 3):705-13 [18310017]
TF
Clp [Q4UZF6, view regulon]
Reported TF sp.
Xanthomonas campestris pv. campestris str.17
Reported site sp.
Xanthomonas campestris pv. campestris str.17
Created by
Dinara Sagitova
Curation notes
-

Experimental Process

pehA-lacZ reporter assays showed that pehA transcription is reduced in the clp mutant. Gel retardation assay shows that Clp binds to the pehA promoter

Transcription Factor Binding Sites


CGTGGATCTGCGCACT
CGTGGATCTGCGCACT

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
CGTGGATCTGCGCACT peh-1
... ... peh-1
Experimental technique details Beta-gal reporter assay - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Visual sequence inspection (nan) - activator monomer