Curation Information

Analyses of binding sequences of the two LexA proteins of Xanthomonas axonopodis pathovar citri.;Yang MK, Hsu CH, Sung VL;Molecular genetics and genomics : MGG 2008 Jul; 280(1):49-58 [18437426]
LexA [Q8PN77, view regulon]
Reported TF sp.
Xanthomonas axonopodis pv. citri XW47
Reported site sp.
Xanthomonas axonopodis pv. citri XW47
Created by
Dinara Sagitova
Curation notes

Experimental Process

Determination of the LexA2-binding site within the X. axonopodis pv. citri lexA2 promoter region was done by using DNA probes corresponding to different regions of the promoter. The results of this analysis indicated that LexA2 binds to a region located between −47 and −22 relative to the lexA2 translation start site. LexA2 binding to this site was confirmed by performing EMSAs. A palindromic sequence was found in this promoter region. Binding specificity was confirmed with site-directed mutagenesis and EMSA analyses. Luciferase reporter assays performed with either the wild-type or mutated lexA2 promoter indicated that LexA2 represses lexA2 expression in X. axonopodis pv. citri.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... lexA XAC1198 XAC1197 doc XAC1195
Experimental technique details EMSA (ECO:0001807) - Experimental technique details Luciferase reporter assay (ECO:0005648) - Experimental technique details Site directed mutagenesis (ECO:0005667) - Experimental technique details Visual sequence inspection (nan) - repressor not specified