RNA dot blot assay confirmed Fur-dependent activation of the ompT expression. EMSA showed binding of Fur to the ompT promoter region. Site-directed mutagenesis showed the binding is specific.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|