EMSAs showed that the mobilities of the exsC, exsD and exoT promoter fragments were significantly retarded in the presence of ExsA. DNase I footprinting experiments were performed to map the promoter regions bound by ExsA. Multiple sequence alignment of 10 ExsA-dependent promoters was performed to identify highly conserved bases. To identify the minimal promoter sequence necessary for ExsA binding a series of probes derived from the exoT promoter was generated.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|