Curation Information

The major catalase gene (katA) of Pseudomonas aeruginosa PA14 is under both positive and negative control of the global transactivator OxyR in response to hydrogen peroxide.;Heo YJ, Chung IY, Cho WJ, Lee BY, Kim JH, Choi KH, Lee JW, Hassett DJ, Cho YH;Journal of bacteriology 2010 Jan; 192(2):381-90 [19933365]
OxyR [Q9HTL4, view regulon]
Reported TF sp.
Pseudomonas aeruginosa UCBPP-PA14
Reported site sp.
Pseudomonas aeruginosa UCBPP-PA14
Created by
Dinara Sagitova
Curation notes

Experimental Process

The electrophoretic mobility shift assay results demonstrated that OxyR may directly regulate the katA gene by binding to the ORE. This was further validated by performing DNase I footprinting experiments. Chromatin immunoprecipitation (ChIP) using an anti-OxyR antibody showed that P. aeruginosa OxyR can bind to the katA promoter region under both noninducing and H2O2-inducing conditions in vivo.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... katA bfrA
Experimental technique details ChIP-PCR (ECO:0005620) - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Visual sequence inspection (nan) - not specified not specified