A putative 16 bp consensus motif for the GlxR binding in the upstream region of the narKGHJI operon was identified in a different study by Kohl et al. (pubmed id: 18573287). To verify specific interaction of GlxR with the putative binding site the following experiments were performed. EMSA showed that in the presence of cAMP, purified GlxR bound to the wild-type narK promoter region. The cAMP-dependent interaction was completely inhibited by introduction of a mutation in the putative binding motif. The wild-type narK promoter–lacZ fusion and the mutant PnarK–lacZ fusion assays confirmed that binding of GlxR is responsible for upregulation of the narK promoter activity.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|