Curation Information

Regulation of the nitrate reductase operon narKGHJI by the cAMP-dependent regulator GlxR in Corynebacterium glutamicum.;Nishimura T, Teramoto H, Toyoda K, Inui M, Yukawa H;Microbiology (Reading, England) 2011 Jan; 157(Pt 1):21-8 [20864477]
GlxR [Q79VI7, view regulon]
Reported TF sp.
Corynebacterium glutamicum R
Reported site sp.
Corynebacterium glutamicum R
Created by
Dinara Sagitova
Curation notes

Experimental Process

A putative 16 bp consensus motif for the GlxR binding in the upstream region of the narKGHJI operon was identified in a different study by Kohl et al. (pubmed id: 18573287). To verify specific interaction of GlxR with the putative binding site the following experiments were performed. EMSA showed that in the presence of cAMP, purified GlxR bound to the wild-type narK promoter region. The cAMP-dependent interaction was completely inhibited by introduction of a mutation in the putative binding motif. The wild-type narK promoter–lacZ fusion and the mutant PnarK–lacZ fusion assays confirmed that binding of GlxR is responsible for upregulation of the narK promoter activity.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... cgR_1269 cgR_1268 cgR_1267 cgR_1266 cgR_1265 cgR_1270
Experimental technique details Beta-gal reporter assay - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Site directed mutagenesis (ECO:0005667) - activator not specified