Computer analysis of the ptxR-promoter region revealed a potential Vfr binding site. EMSAs showed that Vfr binds to this region. Vfr binding site was then localized by performing DNase I footprinting. The protected site contained the putative Vfr binding site. Then Vfr was shown to be an activator of ptxR expression by comparing the results of the lacZ fusion assays in WT and vfr deletion mutant.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|