Curation Information

Regulation of Pseudomonas aeruginosa ptxR by Vfr.;Ferrell E, Carty NL, Colmer-Hamood JA, Hamood AN, West SE;Microbiology (Reading, England) 2008 Feb; 154(Pt 2):431-9 [18227247]
Vfr [P55222, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PA103
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Dinara Sagitova
Curation notes

Experimental Process

Computer analysis of the ptxR-promoter region revealed a potential Vfr binding site. EMSAs showed that Vfr binds to this region. Vfr binding site was then localized by performing DNase I footprinting. The protected site contained the putative Vfr binding site. Then Vfr was shown to be an activator of ptxR expression by comparing the results of the lacZ fusion assays in WT and vfr deletion mutant.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... ptxR ptxS PA2260 PA2261 PA2262 PA2263
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details PSSM site search (ECO:0005659) - activator not specified