Curation Information

Publication

The zinc-responsive regulator Zur and its control of the znu gene cluster encoding the ZnuABC zinc uptake system in Escherichia coli.;Patzer SI, Hantke K;The Journal of biological chemistry 2000 Aug 11; 275(32):24321-32 [10816566]

TF

Zur [P0AC51, view regulon]

Reported TF sp.

Escherichia coli str. K-12 substr. MG1655

Reported site sp.

Escherichia coli str. K-12 substr. MG1655

Created by

Dinara Sagitova

Curation notes

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Experimental Process

Beta-gal reporter assay was used to validate that Zur acts as a repressor. DNA-binding activity of Zur was shown to be metal ion dependent. DNAse I footprinting assays identified a single Zur binding site within znu promoter region.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.