Beta-gal reporter assay was used to validate that Zur acts as a repressor. DNA-binding activity of Zur was shown to be metal ion dependent. DNAse I footprinting assays identified a single Zur binding site within znu promoter region.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|