The inﬂuence of H-NS on bolA expression was assayed during different phases of bacterial growth. RT-PCR showed that in the absence of H-NS, the bolA levels in late exponential phase were significantly higher that in the wt and an increase in the transcript level was about 2.4-fold. EMSAs confirmed that H-NS represses bolA transcription though a direct interaction with the entire bolA promoter region. DNAse I footprinting assay results indicate that H-NS is able to bind simultaneously to several sites within the entire regulatory region of bolA.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|