Curation Information

Publication
A new target for an old regulator: H-NS represses transcription of bolA morphogene by direct binding to both promoters.;Moreira RN, Dressaire C, Domingues S, Arraiano CM;Biochemical and biophysical research communications 2011 Jul 22; 411(1):50-5 [21708124]
TF
H-NS [P0ACF8, view regulon]
Reported TF sp.
Escherichia coli str. K-12 substr. MG1655
Reported site sp.
Escherichia coli str. K-12 substr. MG1655
Created by
Dinara Sagitova
Curation notes
-

Experimental Process

The influence of H-NS on bolA expression was assayed during different phases of bacterial growth. RT-PCR showed that in the absence of H-NS, the bolA levels in late exponential phase were significantly higher that in the wt and an increase in the transcript level was about 2.4-fold. EMSAs confirmed that H-NS represses bolA transcription though a direct interaction with the entire bolA promoter region. DNAse I footprinting assay results indicate that H-NS is able to bind simultaneously to several sites within the entire regulatory region of bolA.

Transcription Factor Binding Sites


TGGTAAAAGA
TCTTTAAATC
TTGTTAAGCT
TATTTAAAGG
TGGTAAAAGA
TCTTTAAATC
TTGTTAAGCT
TATTTAAAGG

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TGGTAAAAGA bolA,
... ... bolA yajG ampG
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - Experimental technique details Visual sequence inspection (nan) - repressor not specified
TCTTTAAATC bolA,
... ... bolA yajG ampG
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - Experimental technique details Visual sequence inspection (nan) - repressor not specified
TTGTTAAGCT bolA,
... ... bolA yajG ampG
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - Experimental technique details Visual sequence inspection (nan) - repressor not specified
TATTTAAAGG bolA,
... ... bolA yajG ampG
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - Experimental technique details Visual sequence inspection (nan) - repressor not specified