Curation Information

Role of sigma54 in the regulation of genes involved in type I and type IV pili biogenesis in Xylella fastidiosa.;da Silva Neto JF, Koide T, Abe CM, Gomes SL, Marques MV;Archives of microbiology 2008 Mar; 189(3):249-61 [17985115]
RpoN [Q9PDH1, view regulon]
Reported TF sp.
Xylella fastidiosa J1a12
Reported site sp.
Xylella fastidiosa J1a12
Created by
Dinara Sagitova
Curation notes

Experimental Process

DNA microarray experiments were used to reveal the global transcriptional profile of X. fastidiosa. Promoter regions of the differentially expressed genes were searched for potential RpoN binding sites by identifying matches with the σ54-binding consensus. Microarray results were validated by qRT-PCR. The transcriptional start site of pilA1 was determined by primer extension analysis. Also, a good consensus for binding of the integration host factor (IHF) was identified between positions −45 and −80.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... XF2542 XF2541 XF2540
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - Experimental technique details Visual sequence inspection (nan) - activator not specified