DNA microarray experiments were used to reveal the global transcriptional profile of X. fastidiosa. Promoter regions of the differentially expressed genes were searched for potential RpoN binding sites by identifying matches with the σ54-binding consensus. Microarray results were validated by qRT-PCR. The transcriptional start site of pilA1 was determined by primer extension analysis. Also, a good consensus for binding of the integration host factor (IHF) was identified between positions −45 and −80.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|