DNase I footprinting experiments were performed on the 453 bp fragment to localize the binding site within the promoter region of ilvB. Gel mobility assays was used to show CodY binds to the ilvB promoter. Beta-gal reporter assay was used to confirm CodY mediated regulation of ilvB.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|