fur::luxAB transcriptional fusion showed that the Fur protein activates its own expression, and the extent of its activation is elevated in the presence of iron chelator added to the growth medium. Specific binding of Fur to the fur promoter was confirmed by performing DNAse I footprinting assays. Mutagenesis of the putative Fur box and a fur(mutant)::luxAB transcriptional fusion confirmed that the interaction between Fur and the protected DNA sequence is essential for autoregulation of the fur gene.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|