Curation Information

Positive regulation of fur gene expression via direct interaction of fur in a pathogenic bacterium, Vibrio vulnificus.;Lee HJ, Bang SH, Lee KH, Park SJ;Journal of bacteriology 2007 Apr; 189(7):2629-36 [17237166]
Fur [P33117, view regulon]
Reported TF sp.
Vibrio vulnificus CMCP6
Reported site sp.
Vibrio vulnificus CMCP6
Created by
Dinara Sagitova
Curation notes

Experimental Process

fur::luxAB transcriptional fusion showed that the Fur protein activates its own expression, and the extent of its activation is elevated in the presence of iron chelator added to the growth medium. Specific binding of Fur to the fur promoter was confirmed by performing DNAse I footprinting assays. Mutagenesis of the putative Fur box and a fur(mutant)::luxAB transcriptional fusion confirmed that the interaction between Fur and the protected DNA sequence is essential for autoregulation of the fur gene.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type