Regulation for both promoters demonstrated by beta-gal assay comparing to a codY-null mutant in aa rich medium. CodY sites were identified by visual comparison to known consensus on DNAse footprinted regions. The functionality of sites was further verified by site-directed mutagenesis, followed by beta-gal assays and DNAse footprinting.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|