MalI binding sites were predicted by analogy to LacI (16 bp palindromic elements) [PMID:2670898]. The whole segment corresponding to the malI-malX intergenic region was mutagenized randomly by PCR and cloned. Colonies showing de-repressed activity in beta-gal assays for malI or malX were sequenced and consistent key mutations revealed the two sites for MalI. Multiple sequence alignments of the region with other Gammaproteobacteria further confirmed the results.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|