Curation Information

Publication
Fur controls iron homeostasis and oxidative stress defense in the oligotrophic alpha-proteobacterium Caulobacter crescentus.;da Silva Neto JF, Braz VS, Italiani VC, Marques MV;Nucleic acids research 2009 Aug; 37(14):4812-25 [19520766]
TF
Fur [A0A0H3C2M1, view regulon]
Reported TF sp.
Caulobacter crescentus NA1000
Reported site sp.
Caulobacter crescentus NA1000
Created by
Dinara Sagitova
Curation notes
-

Experimental Process

Putative Fur-binding sites in the genome of C. crescentus were detected by using a position weight matrix approach. EMSAs validated that Fur binds to the identified promoters.

Transcription Factor Binding Sites


TTTGAGAATGGTTCTCAAT
ATTGCGACAGGTTCTCAAT
GATGAGAATGACACTCAAT
TTTGAGAATGGTTCTCAAT
ATTGCGACAGGTTCTCAAT
GATGAGAATGACACTCAAT

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TTTGAGAATGGTTCTCAAT
... ... CC_0144 CC_0143 CC_0142 CC_0141 CC_0140 CC_0139 CC_0156 CC_0157 CC_0158 CC_0155 CC_0154 hrcA CC_0152 CC_0151 CC_0150 CC_0149 CC_0148 CC_0147 CC_0146 CC_0145
Experimental technique details EMSA (ECO:0001807) - Experimental technique details PSSM site search (ECO:0005659) - not specified not specified
ATTGCGACAGGTTCTCAAT
... ... uppP CC_3606 CC_3607 CC_3608
Experimental technique details EMSA (ECO:0001807) - Experimental technique details PSSM site search (ECO:0005659) - not specified not specified
GATGAGAATGACACTCAAT
... ... CC_3263 CC_3264 CC_3265 CC_3266 CC_3267 CC_3268
Experimental technique details EMSA (ECO:0001807) - Experimental technique details PSSM site search (ECO:0005659) - not specified not specified