Curation Information

ToxR recognizes a direct repeat element in the toxT, ompU, ompT, and ctxA promoters of Vibrio cholerae to regulate transcription.;Goss TJ, Morgan SJ, French EL, Krukonis ES;Infection and immunity 2013 Mar; 81(3):884-95 [23297386]
ToxR [A0A0H3AK48, view regulon]
Reported TF sp.
Vibrio cholerae O395
Reported site sp.
Vibrio cholerae O395
Created by
Elliot White
Curation notes

Experimental Process

Authors used site directed mutagenesis in combination with B-gal assays and EMSAs to show that mutations in the putative site caused both a reduction in expression and a reduction of ToxR binding.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... tcpN/toxT tcpF tcpE tcpT tcpS tcpD tcpR tcpC tcpQ tcpB tcpA tcpJ acfB acfC
Experimental technique details Beta-gal reporter assay - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Site directed mutagenesis (ECO:0005667) - activator monomer