Curation Information

Fur controls iron homeostasis and oxidative stress defense in the oligotrophic alpha-proteobacterium Caulobacter crescentus.;da Silva Neto JF, Braz VS, Italiani VC, Marques MV;Nucleic acids research 2009 Aug; 37(14):4812-25 [19520766]
Fur [A0A0H3C2M1, view regulon]
Reported TF sp.
Caulobacter crescentus NA1000
Reported site sp.
Caulobacter crescentus NA1000
Created by
Dinara Sagitova
Curation notes

Experimental Process

Putative Fur-binding sites in the genome of C. crescentus were detected by using a position weight matrix approach. EMSA validated that Fur binds to its target promoter region. LacZ reporter assays validated Fur-mediated expression regulation. Site-directed mutagenesis of Fur-binding sites confirmed specific binding. DNase I footprinting was used to locate Fur binding sequence.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... CC_3529 CC_3528 sdhA sdhB CC_3525 CC_3524 CC_3523 CC_3530 CC_3531 CC_3532 CC_3533 CC_3534 CC_3535 CC_3536 CC_3537 CC_3538 CC_3539
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details PSSM site search (ECO:0005659) - Experimental technique details Site directed mutagenesis (ECO:0005667) - activator not specified