Curation Information

The lipopolysaccharide modification regulator PmrA limits Salmonella virulence by repressing the type three-secretion system Spi/Ssa.;Choi J, Groisman EA;Proceedings of the National Academy of Sciences of the United States of America 2013 Jun 4; 110(23):9499-504 [23690578]
PmrA [Q8Z1P4, view regulon]
Reported TF sp.
Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S
Reported site sp.
Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S
Created by
Dinara Sagitova
Curation notes

Experimental Process

PmrA binding to the ssrB promoter was confirmed by performing DNase I footprinting, chromatin immunoprecipitation, and EMSA analyses. ssrB-lacZ reporter fusion showed PmrA-dependent regulation of the ssrB expression.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... ssrB ssrA STM14_1685 orf242 ssaB ssaC ssaD ssaE STM14_1692 STM14_1693 STM14_1694 sscA sseC sseD sseE sscB sseF sseG ssaG ssaH ssaI ssaJ STM14_1706 ssaK ssaL ssaM ssaV ssaN ssaO ssaP ssaQ ssaR ssaS ssaT ssaU STM14_1719 valW valV
Experimental technique details Beta-gal reporter assay - Experimental technique details ChIP-PCR (ECO:0005620) - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Site directed mutagenesis (ECO:0005667) - repressor not specified