Curation Information

Publication
The zinc-responsive regulator Zur controls expression of the coelibactin gene cluster in Streptomyces coelicolor.;Kallifidas D, Pascoe B, Owen GA, Strain-Damerell CM, Hong HJ, Paget MS;Journal of bacteriology 2010 Jan; 192(2):608-11 [19915027]
TF
Zur [Q9L2H5, view regulon]
Reported TF sp.
Streptomyces coelicolor A3(2)
Reported site sp.
Streptomyces coelicolor A3(2)
Created by
Dinara Sagitova
Curation notes
-
External databases
ArrayExpress (E-MAXD-56) http://www.ebi.ac.uk/arrayexpress/arrays/E-MAXD-56/.

Experimental Process

Zur regulon was identified by microarray analysis. Binding of Zur to its target promoters was validated by EMSAs. qPCR was used to confirm Zur-mediated transcription regulation.

Transcription Factor Binding Sites


ATGATTATCATTTCTA
ATGAATGTCGTTTTCT
TTGAACATGATTTTCA
ATGATTATCATTTCTA
ATGAATGTCGTTTTCT
TTGAACATGATTTTCA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
ATGATTATCATTTCTA SCO7682,
... ... SCO7682 SCO7681 SCO7680 SCO7679 SCO7678 SCO7677 SCO7676 SCO7683 SCO7684 SCO7685 SCO7686 SCO7687 SCO7688 SCO7689 SCO7690 SCO7691
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details PSSM site search (ECO:0005659) - Experimental technique details qPCR [quantitative real-time] (ECO:0005660) - repressor not specified
ATGAATGTCGTTTTCT SCO7676,
... ... SCO7676 SCO7675 SCO7674 SCO7673 SCO7677 SCO7678 SCO7679 SCO7680 SCO7681
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details PSSM site search (ECO:0005659) - Experimental technique details qPCR [quantitative real-time] (ECO:0005660) - repressor not specified
TTGAACATGATTTTCA SCO0475,
... ... SCO0475 SCO0476 SCO0474 SCO0473 SCO0472 SCO0477 SCO0478 SCO0479 SCO0480
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details PSSM site search (ECO:0005659) - Experimental technique details qPCR [quantitative real-time] (ECO:0005660) - repressor not specified