Curation Information

The quorum sensing regulator HapR downregulates the expression of the virulence gene transcription factor AphA in Vibrio cholerae by antagonizing Lrp- and VpsR-mediated activation.;Lin W, Kovacikova G, Skorupski K;Molecular microbiology 2007 May; 64(4):953-67 [17501920]
HapR [A0A0H3Q915, view regulon]
Reported TF sp.
Vibrio cholerae C6706
Reported site sp.
Vibrio cholerae C6706
Created by
Elliot White
Curation notes

Experimental Process

Researchers used an EMSA to demonstrate both that HapR binds the AphA promoter, and that HapR binding inhibits VpsR binding at their overlapping site. HapR's repression of AphA was both documented in previous studies, and demonstrated with a B-gal assay.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type