Curation Information

Identification of the TcpP-binding site in the toxT promoter of Vibrio cholerae and the role of ToxR in TcpP-mediated activation.;Goss TJ, Seaborn CP, Gray MD, Krukonis ES;Infection and immunity 2010 Oct; 78(10):4122-33 [20679441]
TcpP [Q93TT6, view regulon]
Reported TF sp.
Vibrio cholerae O395
Reported site sp.
Vibrio cholerae O395
Created by
Elliot White
Curation notes

Experimental Process

Based on previous DNAse footprinting data (Krukonis et al 2003 Mol. Cell). Introduce transversions on binding site using site-directed mutagenesis. For each, they test binding with EMSA and expression with beta gal.

The paper reports that TF forms complex with other proteins for binding with reported sites.

The manuscripts provides differential data for binding in the presence or absence of ToxR, and show that tcpP-site mutations do not affect ToxR binding.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... tcpN/toxT tcpF tcpE tcpT tcpS tcpD tcpR tcpC tcpQ tcpB tcpA tcpJ acfB acfC
Experimental technique details Beta-gal reporter assay - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Site directed mutagenesis (ECO:0005667) - activator dimer