Based on previous DNAse footprinting data (Krukonis et al 2003 Mol. Cell). Introduce transversions on binding site using site-directed mutagenesis. For each, they test binding with EMSA and expression with beta gal.
The paper reports that TF forms complex with other proteins for binding with reported sites.
The manuscripts provides differential data for binding in the presence or absence of ToxR, and show that tcpP-site mutations do not affect ToxR binding.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|