Curation Information

Virulence regulator AphB enhances toxR transcription in Vibrio cholerae.;Xu X, Stern AM, Liu Z, Kan B, Zhu J;BMC microbiology 2010 Jan 6; 10(1):3 [20053280]
AphA [H9L4T0, view regulon]
Reported TF sp.
Vibrio cholerae C6706
Reported site sp.
Vibrio cholerae C6706
Created by
Elliot White
Curation notes

Experimental Process

Binding and AphB-mediated expression of the toxR promoter demonstrated with EMSA and lux reporter using three different-sized toxR promoter fragments. Indirect effects on toxR regulated genes also assessed.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... toxR toxS VC0395_A0503 VC0395_A0502 htpG
Experimental technique details EMSA (ECO:0001807) - Experimental technique details Luciferase reporter assay (ECO:0005648) - activator dimer