Microarray analysis was done to identify Zur-regulated genes. Microarray data was validated by real-time RT-PCR. EMSAs validated that Zur bound the promoters of the identified genes. The effect of Zur on the promoter activity of znuCB, znuA and ykgM-rpmJ2 was further validated by constructing lacZ reporter fusion analyses. DNase I footprinting assay was used to precisely determine the Zur binding sites on its target promoters.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|