Curation Information

Molecular Characterization of Direct Target Genes and cis-Acting Consensus Recognized by Quorum-Sensing Regulator AphA in Vibrio parahaemolyticus.;Sun F, Zhang Y, Wang L, Yan X, Tan Y, Guo Z, Qiu J, Yang R, Xia P, Zhou D;PloS one 2012; 7(9):e44210 [22984476]
AphA [Q87L53, view regulon]
Reported TF sp.
Vibrio parahaemolyticus RIMD 2210633
Reported site sp.
Vibrio parahaemolyticus RIMD 2210633
Created by
Elliot White
Curation notes

Experimental Process

Researchers used a B-Gal assay and primer extension assay to determine that AphA represses the OpaR promoter. They then used a DNase I assay to show that AphA protects a region within the OpaR promoter; furthermore, an EMSA of the OpaR promoter showed that in-vitro binding. The site described by the authors derives from the consensus site ATATGCA-N6-TGCATAT

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... VP2516 VP2515 VP2514 VP2517
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Primer Extension assay (ECO:0005657) - repressor dimer